Stored the kit at Room Temperature (RT)
CHANGYU Viral RNA Mini Kits provide the fastest and easiest way to purify viral RNA for reliable use in amplification technologies. Viral RNA can be purified from plasma (treated with anticoagulants other than heparin), serum, and other cell-free body fluids. Samples may be fresh or frozen, but if frozen, should not be thawed more than once. Repeated freeze–thawing of plasma samples will lead to reduced viral titers and should be avoided for optimal sensitivity. Cryoprecipitates accumulate when samples are subjected to repeated freeze–thaw cycles. This may lead to clogging of the membrane when using the vacuum protocol. CY Viral RNA Mini Kits are for general use and can be used for isolation of viral RNA from a wide variety of viruses, but the performance cannot be guaranteed for every virus. CY Viral RNA Mini Kits represent a well-established technology for general-use viral RNA preparation.
The kit combines the selective binding properties of a silica based membrane with the speed of microspin or vacuum technology and is highly suited for simultaneous processing of multiple samples. The sample is first lysed under highly denaturing conditions to inactivate RNases and to ensure isolation of intact viral RNA. Buffering conditions are then adjusted to provide optimum binding of the RNA to the silica membrane, and the sample is loaded onto the Mini spin column. The RNA binds to the membrane, and contaminants are efficiently washed away in two steps using two different wash buffers. High-quality RNA is eluted in a RNase-free water. The purified RNA is free of protein, nucleases, and other contaminants and inhibitors.
1. Rapid isolation of high-quality, ready-to-use RNA
2. No organic extraction or alcohol precipitation
3. Consistent, reproducible result
4. Complete removal of contaminants and inhibitors