DNA isolation kits play a crucial role in isolating high quality and high quantity deoxyribonucleic acid not only from tissues but also from cells. These kits aid users greatly in preparing medium-scale as well as large-scale disinfected genomic DNA that ranges in size from 50 kb to 150 kb. They work effectively in getting rid of contaminating proteins as well as deoxyribonucleic acid not only from tissues but also from an extensive variety of biological samples, including cultured cells, gram-negative bacteria, yeasts, as well as from the tail of mice.
Tissue DNA Isolation kits have been in use to separate DNA from a wide diversity of starting materials, as well. The isolated DNA is appropriate to use in a variety of molecular biology applications, including:
- Genomic Southern blotting
- Restriction digestion
- PCR or long PCR
Some of the notable features, as well as benefits of using Tissue DNA Isolation kits, include:
- Users will be capable of obtaining amplified yields of DNA quickly, usually, within two and a half hours.
- Researchers will be greatly benefitted from an easy, simple procedure when compared to using other methods, such as column-based procedures.
- Using the kits will boost safety as well as convenience.
- With the kits, users will be capable of eliminating the use of anion-exchange columns, chaotropic salts, and risky organic solvents.
Above all, these DNA Isolation kits for tissues are capable of reducing the purification time considerably. Moreover, they come included with all the required reagents for isolating DNA from tissues. Some of the required components that come with these kits include:
- Proteinase K Solution
- Cellular Lysis Buffer
- Protein Precipitation Solution
- RNase Solution
Each set of the tissue DNA Isolation Kit is tested for the nonexistence of DNase contagion. These kits will usually undergo function tests to cleanse DNA from bovine liver, followed by precise intensification with the Expand High Fidelity PCR System.
The Trizol Total RNA isolation protocol is considered the best procedure, as it is appropriate for all types of tissues from an extensive variety of animals, blood samples, as well as plant species. All isolation processes are carried out at room temperature without ice and Diethyl pyrocarbonate-treated water. The RNA precipitates with lithium chloride are used for improved stability of the Ribonucleic acid preparation and development of cDNA synthesis.
The Trizol Total RNA isolation protocol is designed for small as well as for large tissue samples, with the tissue volume ranging from 10 μl to 200 μl, which usually yield the total RNA of about 10 μg to 500 μg. In this method, the extraction of RNA is usually conducted by making use of either a solid phase method or a phenol-choloroform method. However, the phenol-choloroform method is considered highly versatile, as it is adapted easily to diverse tissues that range from whole organs to sub-millimeter biopsy punches.
The major benefit of using the Trizol Total RNA isolation is that it will not degrade RNA. Moreover, RNases can be easy to eliminate and reproducible removal of high-quality RNA from significant biological samples will be attainable with great care and suitable countermeasures.