CHANGYUZeroBackgroundpTOPO-TACloningKit

CHANGYU pTOPO-TA Simple Kit

1. Whether blunt end or A-end PCR product can complete the connection instantly; 

2. Without ice bath and heat shock, complete transformation within 5 minutes at RT; 

3. From the connection to the entire coating plates only 20 minutes;

4. No self-connection, zero background, no blue white screening, nearly 100% positive;

5. Can connect up to 10kb fragments, is the easiest, fastest TOPO vector;

Product Manual 

 

Cat# Size Price ($) Bulk order
CYCV1501 20 apps 89 Contact
CYCV1502 80 apps 309 Contact

 

Storage

-20 °C, stable for 12 months.

 

Description

The Zero Background TA Simple Topoisomerase Cloning Kit is designed for fast cloning of DNA fragments up to 10 kb generated by Taq DNA polymerase which introduce single 3’-A overhang to the end of the DNA product. DNA fragments generated by restriction digestion or obtained by mechanical shearing can also be effectively cloned after end polishing using Taq DNA polymerase. It utilizes DNA strand transfer activity of Viccinia virus topoisomerase I. Vaccinia virus DNA topoisomerase I forms a 3’-phosphoryl intermediate with the plasmid vector containing cleavage recognition motif of 5’CCCTT↓. Covalently bound topoisomerase I then transfer the incised vector DNA strand to the DNA fragment to be cloned with free 5’-OH terminuses. This transferring reaction is rapid and reproducible. The cloning vector pTOPO-TA Simple included in this kit are high copy number plasmids engineered to tolerate mild toxic genes. Regions flanking the cloning site of pTOPO-TA Simple vectors do not contain any common restriction sites which eliminates possible redundancy of restriction sites.

 

Features

1. Whether blunt end or A-end PCR product can complete the connection instantly; 

2. Without ice bath and heat shock, complete transformation within 5 minutes at RT; 

3. From the connection to the entire coating plates only 20 minutes;

4. No self-connection, zero background, no blue white screening, nearly 100% positive;

5. Can connect up to 10kb fragments, is the easiest, fastest TOPO vector;

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